Clinical / Scientific - Bioinformatics NGS Data Analyst

Position Details:

Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Scientist to join their expanding team.

Job Title: Scientist (Immunology, Cell Culture, Genomics)

Duration: 6 months contract, extendable up to 36 months

Location: Cambridge, MA

Note:

The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.

You may participate in the company group medical insurance plan

Job Description:

Position Summary:

We are seeking a scientist to join our Systems Immunology Lab in Pharmaceutical's Inflammation & Immunology Research Unit.

The successful candidate will be responsible for generating and processing single-cell, spatial, and functional genomics data, as well as supporting standard immunoprofiling activities such as cell culture and flow cytometry.

Position Responsibilities:

Follow existing lab standard operating procedures to:

Participate in sample collection of murine and human tissues, including the preparation of tubes, interfacing with in vivo scientists to retrieve samples, isolation of primary immune cells, and standard mammalian cell culture

Participate in sample preparation of murine and human tissues, including tissue fixation, dissociation, sectioning, and antibody staining

Generate spatial and single-cell data via a number of commercial workflows, including 10x Genomics and Parse Technologies

Interpreate quality control metrics for spatial, single-cell and cytometry experiments

Organizational Relationships:

Reports to a scientific director of the Systems Immunology Lab

Collaborates with and supports several multi-disciplinary experimental teams on a per-project basis

Education And Experience:

MA + 3 years of experience in Biology, Immunology, or a related field

Technical Skills Requirements:

3+ years of hands-on experience with standard immunoprofiling technologies, including

Flow Cytometry

Single-cell library preparation (ideally 10x Genomics or Parse Technology)

Preparation and loading of Illumina sequencing libraries

Bonus: generation of spatial transcriptomics data (any technology)

3+ years of experience with primary immune cells, including

Primary immune cell isolation

Maintenace of immune cell lines

Cell culture and in vitro stimulation of primary immune cells

Bonus: experience with CRISPR mediated functional genomics, including electroporation of primary immune cells

Physical Position Requirements:

Typical bench work, including sitting, pipetting, lifting <10lb items

Interview Process:

1st Teams screen (Camera-On)

2nd Onsite (2hr with Stakeholders)

Duration:

6-months

Minimum Education:

MA + 3 years of experience in Biology, Immunology, or a related field